• Dr. Claudio Vasquez investigates the resistance of microorganisms in the Antarctic to tellurite, a derivative of metallic tellurium, which is very harmful and toxic to the environment.

Tellurium is a very scarce element in the earth's crust and its biological role is unknown to date. Although in its elemental state (Te ° metallic tellurium) it exhibits no toxicity, some of its derivatives, such as tellurite, are highly damaging to most bacteria.

In this context, Dr. Claudio Vasquez, professor at the Faculty of Chemistry and Biology at the U. of Santiago will be in charge of the Fondecyt project: "Antarctic tellurite-resistant bacteria: new mechanisms of resistance",  for three years.

The academic works with bacteria isolated in Antarctica, in order to analyze how the microorganisms react in a cold context in contact with the toxic. For the research, 800 toxic- resistant microorganisms, coming from 100 different Antarctic samples, were isolated. "Of all the samples, we chose four which showed greater resistance to unveil the defense mechanisms they use," the researcher says.

Dr. Vasquez adds that "over the years, we have identified proteins that help the cell to remove this toxic and, therefore, we think that these bacteria that have received more stress by being in extreme conditions at the Antarctic, could exhibit more sophisticated mechanisms. Our goal is to find new genes that encode novel proteins involved in the defense against these toxics.” the expert says.

As a projection of this study, the introduction of new genes into plants that grow in tellurium- contaminated environments, such as some mining areas, is expected. This would help the plants so that they can eliminate the polluting elements.

Pollution

The tellurite, produced as a result of industrial pollution, drains in groundwater by inhibiting or eliminating microorganisms that might be beneficial. It affects bacteria, fungi, plants and animals. For this reason, it is important to control the discharges which come from industrial exudates containing tellurium.

Tellurium is the molecular basis of solar cells that collect energy; therefore, any accident that might happen with these solar panels could release toxic elements.

• Universidad de Santiago’s Innovo Center was awarded these funds to run the Flexible Allocation Seed Grant Fund for four years in order to accelerate the development of innovative scientific and technological business ventures of international impact.
• “This grant is in recognition for the work done by the Center’s Business Incubator, which has dedicated itself to promote the innovation and entrepreneurship culture and has helped to create new technology-based companies,” Innovo’s Director said.

In order to strengthen scientific and technological business ventures, the Chilean Economic Development Agency (CORFO, in Spanish) awarded Universidad de Santiago’s Innovo Center 2,600 million pesos to run the Flexible Allocation Seed Grant Fund (SSAF, in Spanish) for four years. These funds will be earmarked for supporting innovative, high-impact start-up companies.

“The objective is to accelerate the development of local scientific and technological business ventures at an early commercial stage which are based on technologies in their last mile of development and have a potential international impact. We have 500 million pesos available for the first year and then, 700 million pesos every year,” Luis Lino, Innovo’s Director, explained.

These resources will be given to scientific and technological entrepreneurs through contestable funding. Those who are interested and meet the requirements will have to apply for it. For the business ventures that are granted SSAF funds, Innovo Center considers a first stage of international commercial validation. For this purpose, Innovo has 10 million pesos available, and for the second stage of commercialization support, it has 50 million pesos. Both stages require co-funding, as entrepreneurs will have to provide 25% of the total cost of the project.

“The contestable funding call will include entrepreneurs with technological projects, as well as other Chilean academic or research centers. The first call is scheduled between August and October this year and the projects will be evaluated according to their innovation degree, teamwork, technology development and their impact on the country,” Lino explained.

In Director Lino’s opinion, this grant is in recognition for the work done by the Center’s Business Incubator, which has dedicated itself to promote the innovation and entrepreneurship culture and has helped to create new technology-based companies.

Today, 33 companies are being incubated in fields like engineering, life science, biomedicine, and information technology with impact on industry. Some business ventures that are worth to mention are the development of a tidal power harvesting equipment, a biotechnological treatment for mining industry liquid wastes, the first electric car developed in Chile and a new energy dissipation system for buildings, among others.


Translated by Marcela Contreras

• Yeast is a type of fungus that is present in multiple fermentation processes required for food. Due to this fact, new applications of yeast are being studied to decontaminate what we eat in a natural way and at low costs. The study is being conducted in the context of an Initiation Fondecyt project led by Dr Francisco Cubillos Riffo, a researcher at the Food Science and Technology Research Center of Universidad de Santiago.
  
   
  
  Yeast plays an essential role in the making of liquors, beer and bread. This fungus allows multiple fermentation processes required for producing foods as we know them. However, the importance of this catalyst goes beyond food production: it has the characteristic of controlling some mycotoxins. Mycotoxins are natural food contaminants that can cause acute poisoning when ingested, inhaled or absorbed.
  
  Through a genetic analysis of the response to the interaction between natural contaminants and yeast, the study led by Dr Francisco Cubillos Riffo seeks to develop new applications that allow decontaminating foods in a natural way and at low costs. 
  
  According to professor Cubillos, food innocuousness is very important in Chile, both for imports and exports. “The laboratory of Food Science and Technology Research Center of Universidad de Santiago (CECTA) is focused on research on food innocuousness. The type of yeast that we are studying now has the characteristic of decontaminating mycotoxin-containing foods,” he added.
  
  Preventing diseases
  
  The importance of this study lies on the need for preventing diseases transmitted by animals, eliminating contamination of human-consumption products. The analysis of this strain and others coming from different places in the country seems to be suitable to find effective applications.
  
  “We will study yeasts of different origins and then we will evaluate them at a genetic level. We will be able to determine what yeast is the one with the highest ability to degrade pollutants or decontaminate food, and at the same time, will conduct genetic studies on the different strains collected,” Dr Cubillos said.
  
  “Many of these toxins manage to enter the food chain and cause damage; therefore, it is necessary to find natural alternatives for decontamination. Yeast is not a chemical product, it is not a toxic treatment, it is cheaper, and most of the time, it is completely innocuous,” he added.
  
  The study will have a broad impact and Dr Cubillos considers the new possibilities as favorable. “Eventually, we could reach the industry and start partnerships with the Faculty of Administration and Economics. Also, with the data collected during the project, mathematical models can be developed to determine the specific behavior of yeasts in stressful environments, what would contribute even more to future studies,” he said.
  
  Translated by Marcela Contreras

• Dr Laura Almendares Calderón, professor at the Technological Faculty of Universidad de Santiago, presented her study “Development of a technology to replace prickly pear skin with a peel to keep the physiological, microbiological and organoleptic properties of the fresh fruit” at the Expo Milano 2015 (Italy). Dr Almendares presented the innovation in an activity devoted to the best sustainable development practices for food security.

Dr Laura Almendares Calderón, professor at the Technological Faculty of our University, carried out a technical visit to the Expo Milano 2015 (Italy) in order to get an insight of the food situation around the world. She was able to see a wide variety of raw materials, manufactured goods, equipment and supplies exhibited by more than one hundred countries. The activity had the presence of leaders from all over the world, like President Michelle Bachelet, who opened the Chilean Pavillion.

In this context, Dr Almendares, director of the FIA-USACH Project, PYT-2012-0033, “Development of a technology to replace prickly pear skin with a peel to keep the physiological, microbiological and organoleptic properties of the fresh fruit”, presented her work at the BSDP Week.

The activity started with an exhibition of photos, porters, brochures and other information material related to this matter. The academic was able to show the results of this Chilean innovative project to people from different countries, at the Urban Center, Galleria Vittorio Emanuele, in downtown Milano, between June 10^th and 13^th.

Chile participated in the competition “Feeding Knowledge”, a program created to contribute to the permanent legacy of the Expo Milano 2015.

With that purpose in mind, a document will be generated containing policies and key recommendations to create an effective knowledge system in the food security field in the Mediterranean Region. The final version of this document will be available in September this year.

Selection of proposals

The proposals submitted by eligible candidates underwent a strict admission control by the International Selection Committee, which is responsible for the final evaluation, using nine pre-established criteria.

The proposals that did not meet one or more of the admission criteria were not considered as “Good Practices in Food Safety.”

The ones that were well evaluated officially became “participating initiatives”, like the work presented by Dr Almendares. 

Her work was included in the priority theme “Food consumption habits: diet, environment, society, economy and health.”

This theme groups all projects which objectives are focused on research activities that evaluate the impact of current diets on the environment, economy, society, culture, health and nutritional sustainability.

This was the only Chilean study presented at the activity and it was registered as ‘9712. Development of methodology to replace prickly pear skin for enriched eatable peel. Chile. 25’.

Translated by Marcela Contreras

• In the context of a research work on isolated microorganisms in the Chilean Antarctica, the research team led by Dr Claudio Vásquez, professor at the Faculty of Chemistry and Biology of Universidad de Santiago, discovered that glutathione reductase is one of the enzymes able to reduce tellurite, a compound which is highly toxic to almost all microorganisms.

Tellurium, a chemical element with symbol Te and atomic number 52, seems to be non-toxic. However, when combined with other elements like oxygen, it produces tellurite, which is very harmful to living organisms.

A research team led by Dr Claudio Vásquez studied the mechanisms that bacteria use against high concentrations of toxic metals. The results of the study were published in the American Society for Microbiology’sjournal Applied and Environmental Microbiology, USA.

This study is part of the Regular Fondecyt Project N° 1130362 “Tellurite-resistant Antarctic bacteria: Unveiling new toxicant resistance mechanisms,” which also inquired into how oxygen is partially reduced with the concomitant generation of reactive oxygen species (ROS) in the cells exposed to a toxicant. Organisms that depend on oxygen to breath live in an oxidative environment that affects their cells. Therefore, to prevent the cell’s structure and chemical composition from being damaged, they have an inner reductive environment,” he explains.

In the Antarctica

To collect the required samples, Dr Vásquez and Dr José Manuel Pérez of Universidad Andrés Bello, went to the Prat and Escudero Antarctic Bases; they visited Deception Island and Fildes Peninsula and travelled on the Almirante Óscar Viel ice-breaker of the Chilean Army.

“As the Antarctic laboratories are well equipped, we were able to process part of those samples. We wanted to isolate the Antarctic microorganisms resistant to the toxic salt tellurite that we had studied years ago at the university laboratory,” Dr Vásquez says. In the samples that they studied, they were able to isolate several tellurite-resistant bacteria.

Tellurite reduction

Dr Vásquez and his team were able to prove that glutathione reductase is responsible for reducing tellurite and, therefore, for the cell’s resistance to this toxicant.

“We purified proteins as of crude extracts of resistant bacteria and we found that a particular enzyme, glutathione reductase, was largely responsible for reducing the toxicant, as it changed it to its non-toxic metallic form,” he says.

“We tested these nanoparticles and we found that they have antibacterial properties, so they can be used to fight pathogenic bacteria that cause disease,” he adds.

It is worth to mention that the studies conducted by Dr Vásquez are eco-friendly, as he uses biosynthesis: He reduces metals by using proteins or cells and not chemical substances. In this way, it is possible to lower expenses and work at environment temperature, avoiding negative impacts on the ecosystem.

Dr Vásquez says that as tellurite is rare in the environment, it has been poorly studied and its properties are not well understood.

The research team is made up of the following members: Dr Benoit Pugin, Fabián Cornejo and Pablo Muñoz-Díaz (biochemists), Claudia Muñoz-Vilagrán, Joaquín Vargas-Pérez (biochemist) and Dr Felipe Arenas.

To read the full paper, search “Glutathione reductase-mediated synthesis of tellurium containing nanostructures exhibiting antibacterial properties” on the web.

Translated by Marcela Contreras

• The study “Application of nanotechnology to develop a new ethylene adsorber oriented to the production of packaging for climacteric fruits,” successfully concluded. The new mechanism will allow delaying the ripening process of Chilean horticultural products exported to countries in Europe, North America and Asia.

Chile is a leading exporting country of horticultural products. As its most important buyer countries are in Europe, North America and Asia, shipping distances pose a challenge with regard to keeping the quality of these products.

In 2012, in order to contribute with a solution to this problem and because of Universidad de Santiago’s vocation to serve the country, the project “Application of nanotechnology to develop a new ethylene adsorber oriented to the production of packaging for climacteric fruits” was started, with the support of the Fund for the Promotion of Scientific and Technological Development (Fondef, in Spanish).

Dr Francisco Rodríguez, professor at the Department of Food Science and Technology, the Packaging Laboratory (Laben, in Spanish) and at the Center for the Development of Nanoscience and Nanotechnology (Cedenna, in Spanish) of Universidad de Santiago, has led the research team.

Ethylene gas control

After four years, the results confirmed the study’s hypothesis to use a packaging system that includes a mechanism to control ethylene gas and delay the ripening process. Ethylene gas controls plant growth and accelerates the maturation process.

“Our goal was to develop ethylene active films based on modified aluminum silicates and polyethylene in order to produce a material that can be used when shipping these products to distant markets,” the researcher said.

In the study, researchers used climacteric fruits like banana, plum and avocado. They had a positive response to the incorporation of an active plastic material based on zeolite, which structure was modified with some metals. “Modified zeolite showed an ethylene removal capacity five times higher than the capacity of non-modified zeolite,” Dr Rodríguez said.

Project closing seminar

The final results of the project led by Dr Rodríguez were presented at a seminar held in Hotel Plaza San Francisco. Representatives of some of the collaborating entities participated in the activity, like Maderas Bravo, Clariant and San Jorge Packaging

In this regard, Sergio Carrillo, Coordinator of the Department of Technology Management of Universidad de Santiago de Chile, said: “The focus now is on technology transfer, but for a long time, it was on research, and the market was out of the university scope. Fortunately, this has changed lately.”

For his part, Dr Rodríguez mentioned the possibility of continuing with this work so as to see the study results in the market, i.e, the use of the film in fruit exports. Up to now, the system works well, but it requires some adjustments to obtain a better product before going to market.

Translated by Marcela Contreras

• The projects supported by the Department of Agrarian Management of the Technological Faculty and the Food Science and Technology Research Center of Universidad de Santiago show important results, like a bio-pesticide based on residual quinoa grains or the potential edible use of this pseudo-cereal leaves.

The projects supported by the Department of Agrarian Management of the Technological Faculty and the Food Science and Technology Research Center of Universidad de Santiago (Cecta, in Spanish), show important progress in their goal of feeding the population in a healthy and equitable way.

The first project, “Biopesticidas en base a saponinas de quínoa” (Bio-pesticides based on quinoa saponins) (FIC 30343624-0) lasts three years and it is being developed in the O’Higgins Region, in Central Chile. It has the purpose of using the residual quinoa grains to generate a natural pesticide for grapevines.

The second project, “Valorización agroindustrial de subproductos de la quínoa” (Agro-industrial valuation of quinoa byproducts) (FIC 30429825-0), lasts three years and it is also being developed in the O’Higgins Region. The goal of this project is to promote the cultivation of quinoa, with new applications. Besides using the grains, they expect to promote the use of the leaves in salads.

The third project, “Habilitación de productores hortícolas de la región Metropolitana para la elaboración de productos IV gama” (Training vegetable producers of the Metropolitan Region in the elaboration of IV range products) (GORE BIP 30442786-0), lasts 18 months and it is the continuation of a project developed by the Cecta researchers in 2011 that tested different protocols to reduce the microbial load in vegetables like lettuces, cabbages and carrots.

Carlos Díaz Ramírez, Professor at the Department of Agrarian Management and Innovation Manager of the projects, explains that the purpose of this project is to train small farmers in the care and safety of all the production and supply chain of vegetables, until the products reach the consumer.

Some of the Cecta scientists involved in the projects are Professor Lina Yáñez Catalán, Dr Claudio Martínez and Dr José Luis Palacios Pino.

• The project, led by Dr. Ricardo Salazar, professor at the Faculty of Chemistry and Biology of the University, aims at decontaminating the water from dyes waste and additives, by using electricity and solar energy.

The textile industry in Chile was born in the mid-nineteenth century and expanded thanks to the measures of protection of the internal market which were implemented at that time. Another factor was the arrival of Palestinian immigrants that gave prosperity to the development of the industry.

However, as all industrial activity, this industry was also a contaminant, due to the use of water in its tasks.

This situation becomes a serious problem when you consider that our country has  supply and drought problems. In this context, Dr. Ricardo Salazar, an academic at the Faculty of Chemistry and Biology at the U. Santiago, is leading the Fondecyt project: "Degradation of dyes in wastewater from the textile industry by electrochemical oxidation technologies.” With this project, he aims to provide a solution for wastewater reuse in this process.

The study comes from a previous work by this expert that consisted in analyzing water decontamination of pesticides used in the wine industry. "The first two projects involved water treatment in the laboratory and comprised a chemical study. Now, however, I proposed the construction of a pilot plant to treat more wastewater from the textile industry”, Salazar said.

The project aims to be a contribution to environmental conflict resolution. This is the vital motivation for this academic, who seeks to decontaminate waters that contain dyes wastes and additives. To achieve this, he will work with electricity and solar energy and without using chemicals.

In addition, Dr. Salazar adds that "laws are becoming more stringent for industries in terms of technology demand and waste disposal rates. Therefore, the industries will have to be prepared. The idea is to step forward and provide an approach to this conflict and be useful in the future. "

Purification Process

The purification process is performed by the hydroxyl radical, which derives from water oxidation. This element reacts with the organic components present in the water, degrades pollutants and transforms the contaminant organic compounds into carbon dioxide.

Some of the steps included in this four-year project are: to finish the work in the laboratory, which aims to observe what happens in the whole process; identify each of the compounds that are produced and, finally, build a pilot plant. In this last stage, the scholar has the direct support of Dr. Julio Romero, project co-investigator who is also a researcher at the Faculty of Engineering of the University.

For Dr. Salazar, the importance of the research that he develops lies, mainly, on the human capital formation and in the "responsibility of changing the image of research in the country. Our work could contribute to the enterprise, the industry and, obviously, the University, as we could get the latest technological equipment to develop the project and internationalize the name of the institution. "

By Marcela González

• “Broccoli’s myrosinase enzyme production and encapsulation for its use as a food supplement” That is the name of the Fondef project recently awarded to Alejandro Angulo, a graduate of Universidad de Santiago. In the VIU line (Valorización de la Investigación en la Universidad, in Spanish), the funds will allow to develop a capsule to prevent different cancers.

It is well known that eating vegetables provides many health benefits; even more: some of them have disease preventive properties. Like broccoli, for example, that according to different studies, can be a natural anticancer agent.

Based on this idea, Alejandro Angulo, Biotechnology Engineer graduated from Universidad de Santiago, submitted the project “Broccoli’s myrosinase enzyme production and encapsulation for its use as a food supplement” to the IV VIU Contest of Fondef (Fund for the Promotion of Scientific and Technological Development) and he was recently awarded the funds. The initiative has the purpose of developing a capsule to enhance the natural ability of the broccoli to prevent different cancers.

Alejandro Angulo, director of the project, explains that this vegetable is able to produce some antioxidant and anticancer compounds called isothiocyanates, like sulforphane, that is highly powerful. The precursor to this compound, the myrosinase enzyme, is found in broccoli. When you chew it, its tissue breaks down, the enzyme and the substrate react and sulforphane is naturally released. “If we have more optimal or high-activity enzymes, we could maximize the content of these anticancer compounds,” the researcher said.

For the above, he proposes to create a capsule containing purified enzyme that, when eaten with broccoli, increases the sulforphane content in the body, and therefore, its anticancer effect. However, the researcher warns that the product “would allow preventing cancer, but it would not be a treatment for cancer.”

The researcher says that the idea of developing this food supplement arose when he was looking for a topic for his dissertation work and contacted Dr. Andrea Mahn. She was working on a Fondecyt project that sought to transform broccoli into a functional food. “I focused on the broccoli enzyme that acts as a catalyst for the chemical reaction that releases the anticancer compound, and aspect that she was not studying. In my dissertation work I was trying to describe this enzyme to then purify it and leave it ready to be used in the product. It was then when we thought of developing a food supplement,” he remembers.

His idea was one of the 12 proposals submitted by Universidad de Santiago that won the last VIU Contest version, a historical record that ranks our University in the first place this year. The study will have the support of the Department of Technology Transfer (DGT, in Spanish) to move forward to the ultimate goal: to develop the product for market.

The project is at its first stage that includes a business plan and a work plan; then it will be evaluated to continue to the second stage: the project implementation. “In the long term, we expect to meet all the project stages and position the product as a recognized brand. The idea is to position the brand and sell our product,” the researcher concluded.

Translated by Marcela Contreras

• A new index is the result of the study conducted by a research team at the Department of Informatics Engineering of Universidad de Santiago, led by Dr Max Chacón. This may be a great contribution to improve early detection and measurement of neurodegenerative diseases, like amyothrophic lateral sclerosis, Alzheimer’s disease, Parkinson’s disease and other alterations in cerebral hemodynamics, including cerebrovascular accidents, and subarachnoid hemorrhages, among others.

Cerebral autoregulation is a mechanism which aims to maintain stable cerebral blood flow, despite of the changes in blood pressure.

In order to measure this mechanism, the Aaslid Tiecks method is widely used, but it is not accurate and sometimes it provides false positives that make difficult to differentiate between healthy and sick subjects.

During the study conducted by academics of our University in partnership with the Department of Cardiovascular Sciences at the University of Leicester (United Kingdom), when the new model was applied to 16 healthy men, promising results were observed. 

This new index uses two parameters that are obtained directly from the response signal of the brain to a decrease in arterial blood pressure caused by the sudden release of bilateral thigh cuffs, and a third parameter that measures the difference between the gradient of this response and the change in arterial blood pressure.

“This new index means an improvement in the whole system. The former index did not allow differentiating between healthy and sick individuals in a correct way. When you see the results of the tests, there is an improvement in the evaluation of healthy subjects. The next challenge is to test the index in pathological cases, to confirm the results already obtained,” Dr Chacón said.

University support

Professor Chacón stressed that the study was “completely conducted at the university.” He also highlighted the support that they received from Universidad de Santiago, as the study was brought forth thanks to the contribution of the Department of Scientific and Technological Research and the Department of Informatics Engineering.

Besides, professor Chacón expressed his gratitude to his work team, made up of Dr José Luis Lara, co-author of the study and professor at the Department of Informatics Engineering, and Dr Ronney Panerai, also co-author of this work and professor at the University of Leicester. The researcher also thanked Dr Gonzalo Acuña and Dr Millaray Curilem, both professors at Universidad de la Frontera, who did not formally take part in the study, but contributed to make this research a reality.

Publication of paper

The study results were published in the paper ‘A new model-free index on dynamic cerebral blood flow autoregulation’, where the new index is proposed. It represents a breakthrough in medicine.

The paper was also published by Plos one, one of the most important scientific journals around the world. According to Dr Chacón, this journal is one of the fastest means to publish, so it provides a way to disseminate the results of his work.

He explained that his work “should have a big impact because it is a useful tool at the service of medicine. As it is useful and shows concrete results, the paper might be cited in several occasions.”

Translated by Marcela Contreras