• By means of a Regular Fondecyt Project, a research team at the Faculty of Chemistry and Biology led by Dr. Manuel Azócar is currently working on the optimization of compounds derived from silver, by adding them anti-inflammatory properties and higher thermal stability and durability for their future use as antibacterial agents.

Besides being a metal used in jewelry, silver has varied applications and properties. One of them is its high electrical conductivity. Another distinctive feature of this element is that its particles are used in creams to treat burns and warts; water purification systems; anti-microbial paints and anti-bacterial compounds.

Regarding this last application, Dr. Manuel Azócar, researcher at the Faculty of Chemistry and Biology of Universidad de Santiago de Chile, is developing new silver-based anti-bacterial agents with anti-inflammatory properties, by means of a Regular Fondecyt Project.

The main objective of this research is to understand and enhance the essential features of these compounds for their future use as broad-spectrum bactericidal agents, with more air and light stability.

“We are interested in optimizing these metallo pharmaceutical agents by adding them higher anti-inflammatory properties, higher thermal stability under light conditions and also in identifying the elements that may have toxic effects on cells,” the researcher said.

Besides, one of the most novel aspects of this study is that it seeks to optimize these compounds to use them in lower doses and give them more air and light stability, because silver is a sensitive metal and gets oxidized easily, getting darker in a short period of time.

At a first stage, the work team has been able to prepare several compounds that have made possible to understand the conditions that allow obtaining more stable and durable products. They have also been able to evaluate these compounds as anti-bacterial agents.

“As of 2015, we have planned to assess the cytotoxicity of these compounds, verify our hypotheses regarding the reduction of toxic effects and make a more detailed analysis of their structural features,” the researcher finally said.

Translated by Marcela Contreras

·         The drugs we use are delivered into our bodies in a short time because they are designed to be adsorbed at the intake and to lose effect some time later. In this field, the first results of a study at Universidad de Santiago are very valuable. The research team looked for an “intelligent” drug-delivery system; i.e. a system for a sustained release of the required dose to increase the drug efficacy. 

To understand the mechanisms that would allow in the future releasing drugs in the best place and at the best time, was one of the goals of the study led by Dr Eduardo Lissi, professor at the Faculty of Chemistry and Biology.

Nowadays, the drugs we use are delivered into our bodies in a short time; i.e., they are designed to be adsorbed at the intake and to lose effect some time later.

The purpose of the study was to understand the factors and cellular processes involved in this type of mechanism and Dr Eduardo Lissi, researcher at Universidad de Santiago, together with the Protein Research Group of the Faculty of Biology of Universidad de la Habana (Cuba) and researchers Alexis Aspee (Universidad de Santiago) and Marco Antonio Soto (Pontificia Universidad Católica de Chile), undertook the project.

“I think this is very interesting: designing “intelligent” systems for a particular effect, placing the carrier in the right place to control there the delivery of the bio-active species,” he says.

He adds that he is very “impressed for the magnitude of the problem and for having the possibility of connecting basic biophysics with its applications, particularly with those related to the potential for elaborating specific drugs especially for a given system.”

According to Dr Lissi, these “intelligent” systems would offer advantages in their application, like designing drugs that are released, “for example, when the host reaches a given temperature and/or a given osmotic gradient.” This would assure a sustained delivery of the dose of the required drug and increase its efficacy.

Another aspect of the study, which is still being worked on, is related to the ability of haemolytic toxins to generate channels that contribute to control cell damage. “This involves and interesting potential to selectively kill cell groups that you want to eradicate,” Dr Lissi says. The name of the project was Fondecyt 1130867, “Studies on the diffusion of small solutes through lipids bilayers in unilamellar liposomes."

Translated by Marcela Contreras

• Researchers at CECTA and DECYTAL at Universidad de Santiago successfully participated in two important scientific gatherings. They were the only Chileans participating in the 27^th International Conference on Yeast Genetics and Molecular Biology and the 02^nd International Specialized Symposium of Yeasts, held in the Italian cities of Levico and Perugia, respectively. Dr Verónica García Mena, Dr Claire Brice, Dr Claudio Martínez Fernández and Dr Francisco Cubillos Riffo presented their works at these conferences.

   

  Researchers at the Food Science and Technology Research Center (CECTA, in Spanish) and the Department of Food Science and Technology (DECYTAL, in Spanish) of Universidad de Santiago were the only Chilean representatives in two important scientific activities held in September, in Italy.

  The team led by Dr Claudio Martínez Fernández and made up of Dr Francisco Cubillos Riffo, Dr Verónica García Mena, and Dr Claire Brice presented the results of different studies conducted at the Applied Biotechnology and Microbiology Laboratory.

  The researchers participated in the 27^th International Conference on Yeast Genetics and Molecular Biology, held between September 06^th and 12^th, in Levico, and the 02^nd International Specialized Symposium of Yeasts, held between September 13^th and 17^th in Perugia, Italy.

  Strengthening links

  The director of CECTA, Dr Claudio Martínez, who is currently on a scientific-cultural exchange at the Instituto de Agroquímica y Tecnología de Alimentos (IATA, in Spanish) in Valencia, Spain, explained that the importance of these conferences lies on the possibility of “Strengthening links with the best researchers in this field worldwide, strengthening joint projects and opening opportunities to new collaborations and projects, as well as evaluating the level and the significance of what we are doing in Chile, that it is certainly very good at an international level.”

  For example, the team of CECTA is working on an international collaboration project with researchers at the IATA that also involves Argentinean researchers. And there is another international project with a French team that also participated in these conferences. This team is collaborating with a Fondecyt postdoctoral project and Dr Claire Brice is in charge of the last phase of this study.

  It is worth to mention that CECTA researchers were the only Chilean representatives in both conferences. According to professor Martínez, this was a surprise. And they were more surprised when they realized that, at a Latin American level, there were a few researchers from Argentina, Brazil and Uruguay. “This places us in a leading position at a national level and allows us to strengthen links with Latin American experts in this field to promote local and regional development,” he said.

  Leaders in yeast studies

  Yeasts were the common denominator in the conferences in which Universidad de Santiago researchers participated. Our university is leader in the area of yeast biotechnology in the country. “In this regard, the contributions made by our institution have turned into products (some of them have already been transferred to the productive sector in Chile and abroad) and specialized human resource training and have generated a worldwide renowned group of experts,” he concluded.

   

  Translated by Marcela Contreras

• With the funding of a Fondecyt Postdoctoral Project 2016, Dr Baptiste Darbois, professor at the Faculty of Science of Universidad de Santiago, will be able to accurately determine how lizards move in granular soils in order to produce results that allow to create a robot able to move easily in different types of soil.

With the funding of a Fondecyt Postdoctoral Project 2016, Dr Baptiste Darbois, professor at the Faculty of Science of Universidad de Santiago, will be able to accurately determine how lizards move in granular soils in order to produce results that allow to create a robot able to move easily in different types of soil. This would mean a significant contribution to robotics.

Based on previous research that found that once lizards dive in the sand, they move by wriggling their bodies and not by using their legs, Dr Darbois will study the interaction between a vibrating elastic structure and the granular environment.

The Fondecyt Postdoctoral project (3160167) is called “Locomoción ondulatoria de nadadores suaves dentro de los medios granulares.”

Experimental challenge

The experimental challenge of the study is to control the movement of grains and the forces they undergo when lizards move. This would help to establish guidelines for developing robots able to adapt themselves to different environments. The way of moving of different animals has inspired engineers in this field.

“We expect the compression produced by lizards’ undulating movements in the desert’s sub-surface to help us to develop efficient robots by incorporating this mechanism,” Dr Darbois explained.

Likewise, professor Darbois intends to develop, in the long term, a robot based on the best features lizards show when moving in a granular environment.

“Through this project, we expect to define the optimal conditions: the dimensions, elasticity, frequency and amplitude of vibrations to move forward in waves through a granular environment,” the researcher said.

The relationship between lizards and the development of robots is not odd; on the contrary, it can benefit technological development and improve people´s quality of life. For example, it can be used in critical situations.

“With regard to its applications, developing robots able to efficiently move in granular environments could help to detect anti-personnel mines in the deserts and find people trapped under avalanches,” Dr Darbois concluded.

Translated by Marcela Contreras

• A research team at the Faculty of Chemistry and Biology led by Dr Claudia Ortiz Calderón developed a device based on Chilean brown algae and pumice stone, which is able to adsorb copper from copper-bearing solutions produced by mining industry.

A research team at the Faculty of Chemistry and Biology led by Dr Claudia Ortiz Calderón developed a device based on Chilean brown algae and pumice stone, which is able to adsorb copper from copper-bearing solutions produced by mining industry.

Dr Ortiz, who is in charge of the Laboratory of Vegetable Biochemistry and Phytoremediation of the university, says that this study is part of the research that they usually conduct on the use of plants for environmental remediation.

Using this new device, it is possible to adsorb copper from copper-bearing streams to recover ions and send them back to the mining process, and clean the waters to use them again. Dr Ortiz says that they are already studying the effectiveness of the bio-filter with other metals, what could attract the interest of different companies.

Basically, the bio-filter is a vertical-flow column that contains three types of brown algae – which are very common in the Chilean coast- and pumice stone arranged in a way that is able to capture copper.

“First, we collect the algae and then, after washing, drying, chopping and screening them to a specific size, we package them together with pumicite or pumice stone that prevents the algae from getting compressed,” Dr Ortiz says.

She emphasizes that, in order no produce the bio-filter, they do not require to collect living biomass or to harvest algae. As they use waste algae and do not pre-treat the biomass, the bio-filter has a low cost of production. 

Patent request

The project started in 2012 and was funded by Corfo (the Chilean Economic Development Agent). It is currently at a protection stage after filing a patent request for the packaging system.

The next step is to continue with the analyses to determine the effectiveness of the bio-filter with other metals.

“We know that the bio-filter works very well for copper and we have also tested other equivalent cations, like zinc, cobalt and cadmium, and they have usually worked quite well too,” Dr Ortiz says.

“Thus, companies or industrial processes generating these elements which are interested in removing or recycling them could also be interested in the filter.”

In this context, the Canadian company Good Harbor that supported the project by conducting the hydraulic study of the columns has already expressed its interest in acquiring the rights of this new product.

Translated by Marcela Contreras

• In this way, Dr. Rodolfo Madrid, from the Faculty of Chemistry and Biology, refers to the Conicyt  funds granted for implementing a research ring that will join top scientists. The challenge is to study the physiological role of the TRP ion channels involved in detecting thermal and pain stimuli, among many other physiological processes. Knowing how these channels work may allow the development of treatments for attacking diseases related to the their malfunction.

Three research groups and a common commitment -understanding the role of TRP channels in heat sensitivity, pain and synaptic plasticity-  define the project led by Dr. Rodolfo Madrid researcher at the Faculty of Chemistry and Biology. His initiative has recently received funds from the "2011 Fourth Contest of Research Rings in Science and Technology" organized by the National Commission for Scientific and Technological Research, CONICYT. (See related article).

"Study of the Physiological role of TRP Channels in Thermotransduction and Synaptic Plasticity" is an associative research project that joins three research groups: two correspond to the University’s laboratory of neurosciences at the Faculty of Chemistry and Biology -one led by Dr. Rodolfo Madrid and the other by Dr. Bernardo Morales-  the third is the Interdisciplinary Center for Neuroscience from Valparaiso, led by Dr. Patricio Orio. The joint challenge is to study the physiological role of various members of a group of membrane proteins: polymodal ion channels of the TRP (Transient Receptor Potential) superfamily in diverse physiological processes. For this purpose, they will have the annual funds of 150 million pesos, for three years.

"We want to study the role of various TRP channels in the electrical response of thermoreceptors of cold and nociceptors against various physiological and pathophysiological situations, in order to develop a mathematical model that could serve as a tool for predicting the electrical behavior of these neurons against modifications -that could be the result, for example, of an inflammatory process or an injury-  and  determining  the role of ion channels in regulating neuronal communication in particular regions of the central nervous system," Dr. Rodolfo Madrid explains.

"TRP channels are part of a large group of proteins involved in the detection of thermal stimuli and nociception, among many other physiological processes, and have recently been associated with learning and memory processes," the specialist says.
 

Advanced human capital

This associative research, will attract and train new high-level human capital. It will also  incorporate doctoral and postdoctoral students and, in this way, the group of neuroscientists at the University’s Faculty of Chemistry and Biology will become stronger.

"Besides, we will work with the collaboration of laboratories that belong to some of the world's best centers in the field, such as the Institute of Neuroscience in Alicante, Cambridge University and John Hopkins University, worldwide reference points in the study of neuroscience. This will not only help the growth and consolidation of  our group, but it will also allow our students go directly to stay in those centers,” the researcher says.

Besides, Dr. Madrid values ​​the grant of this ring project "as a recognition that should extend not only to the members of our group, but to all the neuroscientists at the University. From our point of view, this is the most important project of neuroscience that our institution has received and it means that our work has not been the result of improvisation, " he finally  points out.

• Universidad de Santiago’s interdisciplinary research team, led by Dr. Silvia Matiacevich from the Technological Faculty, is focused on developing an edible film that could increase the shelf life of fresh foods by 30%.

Improving the way of preserving foods has been a permanent concern in food industry. This is the reason why packaging is essential for the quality and shelf life of the product. But this packaging should be in harmony with the environment.

In light of this situation, a sustainable alternative for food packaging has been developed: food covering edible films, which are being widely used and have become a world trend nowadays. At Universidad de Santiago, an interdisciplinary research team is trying to replicate this development, giving value added to different national byproducts.

This initiative will be viable thanks to the Associative Dicyt Project called “Bioactive Coatings for Foods”, which gathers together experts from different faculties of the University.

“We will use food industry byproducts which are considered as dispensable or waste material. We are going to give them a value added by adding antioxidant and antimicrobial components to them in order to increase the shelf life of fresh food products,” Dr. Daniel López says.

Academics from three different faculties gathered for this purpose: Dr. Rubén Bustos, from the Faculty of Engineering (Department of Chemical Engineering); Dr. Diego Venegas and Dr. Marlén Gutiérrez, from the Faculty of Chemistry and Biology (Department of Materials Chemistry); and Dr. Daniel López and Dr. Silvia Matiacevich, from the Technological Faculty (Department of Food Science and Technology), being Dr. Matiacevich the leader of the project.

During the two years scheduled for the project, the researchers plan to study the synergistic effect of this combination of products and they expect to increase food shelf life by over 30%.

Interdisciplinarity

Most of the academics related to this project are part of a larger group created by the end of 2013 called Indi, Asociación de Investigadores por el Desarrollo e Interdisciplinariedad of Universidad de Santiago de Chile, a group of researchers that promotes development and interdisciplinarity at the university.

“All of us have participated in some of these initiatives at some point, seeking for this interdisciplinarity. This is how we have met other people and created contacts. What is good is that more than just admiring the work of others, we have the real possibility of conducting studies together. For this reason, we value this type of projects, as they promote the integration and interdisciplinarity that define a university,” Dr. Matiacevich says.

Translated by Marcela Contreras
 

• The motion patterns typical of volcanoes can be predicted with a high rate of effectiveness as of data sent from the slopes of the Llaima Volcano in La Araucanía Region. This has been possible thanks to the work by Dr. Max Chacón, professor at the Department of Informatics Engineering of Universidad de Santiago, who developed a software program for this purpose. “In Chile, it is essential to increase the knowledge on volcanoes. In this way, we will be able to face emergency situations like eruptions, and even predict them and make timely decisions,” Dr. Chacón said.

Due to its location on the Pacific Ring of Fire, the Chilean territory has the second largest volcanic chain in the world, with more than 2,000 volcanoes, many of them located in the southern part of the country. Although they are considered among the most active volcanoes in Latin America, only 43 of them are being monitored. The main concern about these geological structures is their potential for eruptions and their seismic activity is a key factor in prevention. 

Researchers of Universidad de Santiago, Universidad de La Frontera, and the Southern Andean Volcano Observatory, Ovdas by its acronym in Spanish, conducted the study “Pattern recognition applied to seismic signals from the Llaima Volcano: an analysis of the events’ features”, which is available in the Journal of Volcanology and Geothermal Research.

According to Dr. Max Chacón, professor at the Department of Informatics Engineering of Universidad de Santiago and one of the developers of the program, “Based on the machine learning approach, we developed a piece of software that identifies the type of the seismic signal as of data sent by sensors located on the slopes of the volcano,” with an 80% accuracy in the Llaima Volcano.

“Volcano seismic signals are not related to the earthquakes typical of tectonic plate collisions, as one could deduce from a first interpretation of these phenomena. These movements are specific to volcanoes and they are caused by their distinctive features, like their activity, magma movement, gas movement, stiffness of components, etc.

Previously, the research team had studied the Villarrica Volcano, detecting the existence of three characteristic seismic event patterns: the LP (Long Period) event, which is related to the pressure of gas and other fluids in the conduit; the Tremor, which is related to changes in gas and magma densities; and lastly, the VT (Volcano Tectonic) event, which is associated to the fracture of the solid parts of the volcano or the conduits.

The researchers used the program again in a study on the Llaima Volcano, but they also tried to identify the features of data sent by the sensors in the volcano. Among these data, the amplitude, frequency and phase of the signals, and the way in which they appear together in each seismic signal, were particularly considered.

“With this, we tried to give more information to volcanologists, so that they could identify the signals more easily, also analyzing the way in which these features appeared in seismic events,” Dr. Chacón, an expert in system models, said.

According to Dr. Chacón, one of the most interesting conclusions was the similarity of the results in the two volcanoes, what contradicts the current idea that each volcano has unique seismic movements. The researcher says that for now, the hypothesis is that the similarity is due to the proximity between the two volcanoes; however, this has not been proved.

For the above, there are two potential steps to follow in the context of this research. First, a comparative study on these volcanoes to measure the exact differences between them; and second, the improvement of the program with the purpose of detecting the correlations between these seismic signals at the moment of the eruption.

“In Chile, we live with many volcanoes, so it is essential to increase the knowledge about them. The more we know, the better we will be able to face emergency situations like eruptions, and even predict them and make timely decisions,” Dr. Chacón said.


Translated by Marcela Contreras

• The National Institute of Industrial Property recognized Universidad de Santiago as the second Chilean university at filing the highest number of patent applications in 2014. “Chile has a scientific tradition that places the country at the forefront of the Latin American productivity, and numbers reflect this fact, like the second place reached by Universidad de Santiago (last year).” The award ceremony was held in the context of the World Intellectual Property Day.

Once again, Universidad de Santiago was among the three most outstanding universities in the patent category- a category related to the rights given by the Chilean State to an inventor for the development of a new technology- in the annual award ceremony organized by the National Institute of Industrial Property (Inapi). 

The ceremony was held at Patio Los Naranjos of Universidad de Santiago and it was headed by Katia Trusich, Under Secretary of Economy, Development and Tourism, and Inapi’s NationalDirector, Maximiliano Santa Cruz. The Under Secretary said that last year was a consolidation period for Inapi. And she added that the challenge now is to set out a long-term strategy to allow “the development of industrial property considering the specific requirements of the country with regards to productive development, innovation and business ventures.”

For his part, Inapi’s National Director emphasized that our institution has a very important commitment with regards to patent application processing. “In Chile, universities are doing a good job at patenting and, if they are considered all together, in 2014 they almost doubled the number of patent applications filed, in comparison to the previous year.”

Besides congratulating Universidad de Santiago for its great work and interest in patent matters, she said that our University “is making big efforts to obtain patents, something that should be continued and promoted. Generating new knowledge through scientific activity at universities is an essential tool for economic, social and cultural development.”

“Chile has a scientific tradition that places the country at the forefront of the Latin American productivity, and numbers reflect this fact, like the second place reached by Universidad de Santiago in the national patent application ranking of 2014”, Maximiliano Santa Cruz said.

Luis Magne, Head of the Department of Technology Management of Universidad de Santiago- the unit in charge of processing patent applications of the University, among other tasks- said: “Universidad de Santiago continues to keep its spirit of technological university, so it puts an emphasis on applied research and innovation in order to contribute to the society welfare and to have an impact on Chile and the world.”

According to the information given by the Department of Technology Management, in 2014, Universidad de Santiago filed 23 new patent applications with Inapi, doubling the number of patents filed in 2013. This placed the University in the third position of the ranking that year. In addition, it filed 44 invention and protection patent applications with international agencies.

These patents are related to the fields of Sciences, Engineering, Technology, and Chemistry and Biology, and most of them belong particularly to the areas of Biotechnology, Manufacturing and Aquaculture.

Universidad de Concepción was at the first place in the patent application ranking while Pontificia Universidad Católica was at the third place.

Translated by Marcela Contreras

•  Dr Jenny Blamey, professor at the Faculty of Chemistry and Biology described the 13^th International Thermophiles Meeting as a very important opportunity for the development of research at Universidad de Santiago and in the country. Worldwide prominent figures in microbiology, molecular biology, biochemistry, genetics, biocatalysis and biotechnology, participated in the conference. This is the first time that this global meeting is held in South America and Dr Blamey was in charge of the organization.

   

  In her closing speech, Dr Jenny Blamey, professor at the Faculty of Chemistry and Biology of Universidad de Santiago, evaluated this global meeting as a very important opportunity for the development of research at Universidad de Santiago and in the country.

  Dr Blamey highlighted the scientific importance of this conference that always leaves its imprint on the research centers and the countries where it is held. This also happened in our university and it should be reflected on the country.

  During the activity, worldwide leading scientists in this field presented their top-notch studies.

  These experts in microbiology, molecular biology, biochemistry, genetics, biocatalysis and biotechnology informed about the progress in their specialities, in order to have a better understanding of thermophiles.

  The Thermophiles International Conference is a global meeting held every second year. For the first time the meeting was carried out in South America, under the general supervision of Dr Blamey, and with the support of an efficient team who coordinated different tasks for the organizing bodies: Bioscience Foundation and the Faculty of Chemistry and Biology of Universidad de Santiago.

  The program included sessions on topics like genomics and biochemical processes, keynote lectures and poster presentations.

  International experts like Dr Karl Stetter, Dr Michel Adams and Dr Juergen Wiegel met with students, researchers and Chilean and foreign scientists.

  They shared their questions and their knowledge about the last developments and potential biotechnological applications of thermophiles, which are considered key to science development in our country and the world.

  A space for discussion and proposals

  “For Universidad de Santiago de Chile, hosting and promoting this type of activity is essential, since our purpose as a public, state and complete university is to create, preserve, disseminate and apply knowledge for the welfare of society,” Dr Juan Manuel Zolezzi, President of Universidad de Santiago, said.

  The Thermophiles 2015 International Conference contributed “To our institutional essential work, as it became a space for discussion and proposals, in a multidisciplinary and pluralistic dialogue; on this occasion, in the field of extremophiles, a new important field worldwide, not only for biological sciences but also for industrial biotechnology,” he added.

  For his part, Dr Gustavo Zúñiga, Dean of the Faculty of Chemistry and Biology, highlighted the importance of having been selected to organize the activity and receive these distinguished scientists, and give the students the opportunity to ask questions and talk to them.

  Translated by Marcela Contreras